high stringency buffer (Thermo Fisher)

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Thermo Fisher high stringency buffer
High Stringency Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+stringency+buffer/TRIS-HCL/bio_rxiv__2023__09__04__556039-493-14-35
Average 99 stars, based on 1 article reviews

high stringency buffer - by Bioz Stars, 2026-10

99/100 stars

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Article Title: Characterization of the AeaHP gene and its expression in the mosquito Aedes aegypti (Diptera: Culicidae).
Article Snippet: Thereafter, membranes were washed in low stringency buffer (2 15 min in 2 SSC and 0.1% SDS) and high stringency buffer (2 15 min in 0.5% SSC and 0.1% SDS, 50 C), blocked (SuperBlock, Pierce, Rockford, IL, at room temperature, 1 h), and treated with streptavidinhorseradish peroxidase (1:1200; Pierce) for 15 min. After washing (6 15 min at room temperature in 0.025 M TRIS (pH 7.5), 0.15 M NaCl, and 1% Tween 20), membranes were covered with reagents from a Renaissance Chemiluminescence Kit (NEN, Boston, MA) and exposed to BioMax LS Þlm for 5 to 30 min.

Article Title: Inhibition of Ongoing Influenza A Virus Replication Reveals Different Mechanisms of RIG-I Activation
Article Snippet: Briefly, A549 cells were infected with the chicken egg allantoic fluid PR8 stock at an MOI of 50 in the presence of CHX for 6 h. The cells were washed, trypsinized, and lysed in a buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1.5 mM MgCl 2 , 1% NP-40, 1 mM EDTA, 100 μg/ml Escherichia coli tRNA (Roche), 100 U/ml RNasin (Promega), and 1× EDTA-free protease inhibitors at 4°C for 1 h. Meanwhile, 40 μl protein G Dynabeads (Invitrogen) were conjugated with 3 μg of either mouse IgG1 isotype control (CST) or mouse anti-RIG-I antibody (1C3; Millipore).

Article Title: RNA binding proteins and glycoRNAs form domains on the cell surface for cell penetrating peptide entry
Article Snippet: The washes were all 1 mL per sample, and occurred in series: once with High Stringency buffer (15 mM Tris-HCl pH 7.5, 5 mM EDTA, 2.5 mM EGTA, 1% Triton-X100, 1% Sodium deoxycholate, 0.001% SDS (ThermoFisher Scientific), 1200 mM NaCl, 625 mM KCl), three times with 5% SDS in 0.5x PBS, and then once in 25°C 1x PBS.

Hybridization:

Article Title: Inhibition of Ongoing Influenza A Virus Replication Reveals Different Mechanisms of RIG-I Activation
Article Snippet: Thirty micrograms of total RNA was resolved on a 15% acrylamide-8 M urea denaturing gel, followed by transfer onto Hybond N+ membranes (Amersham) in a Trans-Blot SD semidry transfer cell (Bio-Rad) at 1 mA/cm 2 for 1.5 h. The RNA was cross-linked to the nylon membrane at 120,000 μJ/cm 2 for 1 min in a Stratalinker UV cross-linker (Stratagene). .. The membranes were prehybridized in Ultrahyb hybridization buffer (Ambion) at 42°C for 30 min and hybridized with 1 nM 5′-biotinylated probes against NP-5′vRNA (5′-AAAAATACCCTTGTTTCTACT-3′), NP-5′cRNA (5′-AGTGATTATCTACCCTGCTT-3′), or U6 (5′-GCCATGCTAATCTTCTCTGTATC-3′) at 42°C for 16 h. The membranes were washed 2 times for 5 min each time with a low-stringency buffer (2× SSC [1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate], 0.1% SDS) and 2 times for 10 min each time with a high-stringency buffer (0.1× SSC, 0.1% SDS) at 42°C and developed using a chemiluminescent nucleic acid detection module kit (Thermo Scientific) according to the manufacturer’s instructions. .. The membranes were visualized in a Molecular Imager VersaDoc MP 4000 system (Bio-Rad).

Membrane:

Article Title: Exonization of a LINE1 fragment implicated in X-linked hypohidrotic ectodermal dysplasia in cattle.
Article Snippet: *Section of Genetics and Bioinformatics, Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Groennegaardsvej 3, DK-1870 Frederiksberg C, Denmark.. Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Groennegaardsvej 15, DK-1870, DK-1870 Frederiksberg C, Denmark.. Department of Small Animal Clinical Sciences, Faculty of Life Sciences, University of Copenhagen, Dyrlaegevej 46, DK-1870 Frederiksberg C, Denmark.

Article Title: Three GADD45 isoforms contribute to hypertonic stress phenotype of murine renal inner medullary cells.
Article Snippet: Chakravarty, Devulapalli, Qi Cai, Joan D. Ferraris, Luis Michea, Maurice B. Burg, and Dietmar Kültz.. Three GADD45 isoforms contribute to hypertonic stress phenotype of murine renal inner medullary cells.. Am J Physiol Renal Physiol 283: F1020–F1029, 2002.

Centrifugation:

Article Title: Involvement of MicroRNAs in the Regulation of Muscle Wasting during Catabolic Conditions
Article Snippet: .. Hybridized slides were successively washed in low and high stringency buffer (Ambion) for 30 s and dried by centrifugation (500 × g ). .. Array scanning was carried out using a GSI Lumonics LITE dual confocal laser scanner with a ScanArray Microarray Analysis System (PerkinElmer Life Sciences), and raw images were analyzed with QuantArray Analysis Software (GSI Lumonics).

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